NEXCOPE DIC Observation Set (NE900)
DIC Nomarski set with 4 high NA S-Apo objectives
- Complete DIC upgrade set for the NE900 microscope series
- Contains 4 high quality High NA S-Apo lenses (M25)
- 10x (NA 0.3), 20x (NA 0.5), 40x (NA 0.75), 100x oil (NA 1.4)
- Polarization filter for light field can be rotated and swivelled
- DIC condenser with 3 DIC slots for 10x, 20x / 40x and 100x
- Condenser NA 1.25 for bright field and epi-fluorescence
- Contains 2 adjustable DIC sliders for 10x / 20x and 40x / 100x
Product number:
NX82932
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FAQ - Frequently Asked Questions
For this application we recommend the BRESSER Erudit DLX 40-1000x microscope in combination with a USB camera, e.g. the BRESSER MikroCam 1.3 MP. This solution offers better image quality and flexibility than a simple LCD microscope.
If you want to identify bacteria, it is usually more useful to create a culture. Microscopes are generally only sufficient to determine whether the bacteria are, for example, spherical or rod-shaped.
The Barlow lens is already installed in the eyepiece tube on many devices. It can be pulled out at the upper ridge to use the zoom function.
Possible causes:
- Sample too thick / unsuitable: Use thin samples or fixed smears.
- No cover glass or incorrect thickness: Objectives are optimized for cover glasses.
- Condenser incorrectly adjusted: Do not open the diaphragm too far – otherwise contrast will be low.
- Magnification too high: Rule of thumb: maximum 1000 × objective aperture. Example: With a 40x objective with aperture 0.65, the limit is about 650x.
Product information "NEXCOPE DIC Observation Set (NE900)"
Differential interference contrast is now the standard for many professional life science applications. This method achieves aesthetic microscope images with the highest resolution and a very high contrast. As a contrast method, DIC generates impressive optical cuts, and is particularly useful for uncoloured, unfixed samples and living organisms that cannot be compressed. With preparations such as diatoms or radiolaria, it is possible to obtain aesthetic, textbook-quality images.
The DIC set works according to the Nomarski method. The light is first polarized and divided into two parallel but spatially separated beam paths by a special prism in the condenser. Depending on the material properties or light refraction, interference occurs between these two parallel beam paths when they pass through the sample. This accentuates certain structures in a pseudo 3D relief. Both beam paths are combined in the DIC slider, which is located behind the objectives; the rotatable polarizing filter then enables the desired contrast effect or the direction of the relief effect to be set and perfectly matched to the sample.
In combination with a Nexcope NE900 series microscope, this contrast method provides you with a professional observation instrument for your scientific investigations. It also provides an affordable option for amateur enthusiasts. With the Nexcope DIC, you receive a complete, pre-configured set that you can use without any prior specialist knowledge. Unlike phase contrast, adjustments are not required during the installation process. The DIC set is ready for use in a few simple steps, and the S-Apo objectives can also be used for bright field, dark field or polarization applications. While observing with the NE900 series microscope, you can switch directly between DIC, bright field, polarization or epi-fluorescence.
The DIC set works according to the Nomarski method. The light is first polarized and divided into two parallel but spatially separated beam paths by a special prism in the condenser. Depending on the material properties or light refraction, interference occurs between these two parallel beam paths when they pass through the sample. This accentuates certain structures in a pseudo 3D relief. Both beam paths are combined in the DIC slider, which is located behind the objectives; the rotatable polarizing filter then enables the desired contrast effect or the direction of the relief effect to be set and perfectly matched to the sample.
In combination with a Nexcope NE900 series microscope, this contrast method provides you with a professional observation instrument for your scientific investigations. It also provides an affordable option for amateur enthusiasts. With the Nexcope DIC, you receive a complete, pre-configured set that you can use without any prior specialist knowledge. Unlike phase contrast, adjustments are not required during the installation process. The DIC set is ready for use in a few simple steps, and the S-Apo objectives can also be used for bright field, dark field or polarization applications. While observing with the NE900 series microscope, you can switch directly between DIC, bright field, polarization or epi-fluorescence.
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Accessories
BRESSER MikroCam II 20 MP 1'' Microscope Camera
€1,059.90*
The BRESSER MikroCam II 20 MP 1’’ microscope camera is the professional solution when it comes to microscope imaging.
This camera has a big 1’’ sensor (image area 13.06 x 8.8 mm); this is the biggest sensor of the MikroCam camera range. With this camera it is possible to document roughly the same field of view as it can be seen through the eyepiece (e.g. when using a 10x / 22mm). Other models will cover only 50% or less of the eyepiece field of view.
The Sony IMX183 CMOS-sensor is very sensitive and has extremely low image noise, which makes this camera especially suitable for contrast methods like dark field or phase contrast.
The MikroCam Lab II software comes with a wide range of professional functions, e.g. measurements in live view (calibration slide needed), mosaic, Focus-Stacking (for improved depth of field). All automatic functions (exposure time, gain, white balance etc) can be adjusted manually as well.
Due to the big sensor size the camera will not work with all microscope models. It might be possible that the optics will not illuminate the full sensor, so you experience vignetting. Usually this can be resolved by using the optional 1x optical adapter (item no. 5914001).
Please note that this camera model is not suitable for the stereo microscope BRESSER Science ETD-201. It will cover only a small field of view.
SPECIFICATIONSRobust metal housingProfessional software suite including measurements, dark and bright field calibration, focus stacking, mosaic and moreSave images and video, including time lapsecompatible to most microscopesHigh speed USB 3.0 connection for high frame rateModern Sony® IMX183 Sensor 13,06 x 8,8 mm (1'')1/4’’ connectorC-Mount adapter, compatible with c-mount lenses
SYSTEM REQUIREMENTSWindows 10 or 11Intel i3, i5, i7 or i9 CPU from the 6nd generation orAMD Ryzen CPUOptical Disk Drive or internet connection for software downloadUSB 3.0, 3.1
SCOPE OF DELIVERYcamera bodyUSB 3.0 cable23.2 mm, 30 mm und 30.5 mm adapterSoftware for imaging and processing (Windows compatible)
BRESSER MikroCamII 9MP 4K 1'' Microscope Camera
€1,479.90*
Capture 4K video with the BRESSER MikroCamII 9MP 4K microscope camera. The video can be up to 30 fps depending on the PC used. The camera uses a global shutter, which captures moving objects without distortion. The big image sensor (14.13 x 7.45mm) captures a big field of view. The high light sensitivity of the Sony® IMX305 CMOS-sensor produces excellent images even when using darkfield or phase contrast.The BRESSER MikroCamLabII software includes a variety of useful functions, e.g. measuring in live mode (optional calibration slide needed), mosaic/stitching, focus stacking (for extended depth of field). All camera parameters (exposure time, gain, white balance) can be set manually.Mosaic and focus stacking can be done in live mode, which gives instant results even for unexperienced users. The method is also much faster than the normal procedure where a set of images is rendered afterwards. To use the mosaic function in live mode, a crosstable / mechanical desk is highly recommended to move the sample.The big camera sensor is not compatible with all microscope models. It might lead to vignetting (dark edges). To compensate this, a 1.0x optical adapter (item code 5914001) is recommended.This camera will not work with the ETD-201 stereo microscope as the microscope will illuminate only a part of the sensor. For this microscope, we recommend the model MikroCamII 12MP item code 5914912.
SPECIFICATIONSmodern Sony® IMX305 sensor 14,13mm x 7,45 mm (1'')8,85 MP resolution (4096x2160 pixels)software included, which contains measuring, focus stacking, mosaic / stitching for results with bigger fields or extended focus depthcapture images and video, incl. slow motion and timelapse4K video with up to 30 fpsbig field of viewPrecisely machined metal housingcompatible with numerous microscope modelswith c-mount connector, which is widely used.Compatible with c-mount lenses.1/4" connector, for photo tripods, clamps etc.Highspeed USB 3.0 port
SYSTEMANFORDERUNGENWindows 10 oder 11Intel i3, i5, i7 oder i9 Prozessor ab 6. Generation bzw.AMD Ryzen ProzessorOptisches Laufwerk oder Internetverbindung für SoftwaredownloadUSB 3.0, 3.1
SCOPE OF DELIVERYcamera bodyUSB 3.0 cable3 adapters: 23.2mm 30.0 mm and 30.5 mmsoftware CD (Windows)
BRESSER MikroCamII 5MP HIS Microscope Camera
€1,149.90*
The BRESSER MikroCamII 5MP HIS with the light sensitive and low-noise Sony® IMX264 sensor is perfectly suited for low light applications. The camera uses a global shutter, which captures moving objects without distortion.
With sufficient lighting, the camera is also capable of ultra short exposures as low as 1/50.000 second. In this mode, the camera can virtually freeze fast moving objects.
The BRESSER MikroCamLabII software includes a variety of useful functions, e.g. measuring in live mode (optional calibration slide needed), mosaic/stitching, focus stacking (for extended depth of field). All camera parameters (exposure time, gain, white balance) can be set manually.
Mosaic and focus stacking can be done in live mode, which gives instant results even for unexperienced users. The method is also much faster than the normal procedure where a set of images is rendered afterwards. To use the mosaic function in live mode, a crosstable / mechanical desk is highly recommended to move the sample.
The big camera sensor is not compatible with all microscope models. It might lead to vignetting (dark edges). To compensate this, a 1.0x optical adapter (item code 5914001) is recommended.
This camera will not work with the ETD-201 stereo microscope as the microscope will illuminate only a part of the sensor. For this microscope, we recommend the model MikroCamII 12MP item code 5914912.
SPECIFICATIONSmodern Sony® IMX264 sensor 8,45mm x 7,07 mm (2/3'')5 MP (2448x2048)software included, which contains measuring, focus stacking, mosaic / stitching for results with bigger fields or extended focus depthcapture images and video, incl. slow motion and timelapse1/4" tripod connectorPrecisely machined metal housingcompatible with numerous microscope modelswith c-mount connector, which is widely used. Compatible with c-mount lenses.1/4" connector, for photo tripods, clamps etc.Highspeed USB 3.0 port
SYSTEM REQUIREMENTSWindows 10 or 11Intel i3, i5, i7 or i9 CPU from the 6nd generation orAMD Ryzen CPUOptical Disk Drive or internet connection for software downloadUSB 3.0, 3.1
SCOPE OF DELIVERYcamera bodyUSB 3.0 cable3 adapters: 23.2mm 30.0 mm and 30.5 mmsoftware CD (Windows)
BRESSER MikroCamII Microscope Camera 12MP
€819.90*
The BRESSER MikroCamII microscope cameras help to make photos and videos with a microscope very easy. The modern Sony® CMOS sensors give brilliant colours with very low noise and is the perfect choice for any application where true natural colours are important. The included imaging software has multiple adjustments for the capture of still images and videos. The integrated measuring* routine allows for a measurement in the camera live image. The software can be operated in the following languages: German, English, French, Polish, Russian, Turkish, Chinese. The camera housing includes the c-mount adapter thread. For use with 23.2 mm adaptations, we recommend the optional, variable 0,5x reducer (item no. 5914005). For special applications, the camera can also be mounted using the 1/4'' camera tripod thread. The BRESSER MikroCamII are equipped with a USB 3.0 port, compared to the MikroCam SP. This enables up to 8 times faster data transfers to the PC compared with the USB 2.0 standard. The camera is able to deliver more than 25 frames per second (depending on resolution, exposure time and PC performance). This is especially important when observing live microorganisms. In addition to this, the camera is exceptionally sensitive. Even in low light conditions (high magnifications, contrast methods) the camera can be operated with short exposure times.
FEATURESHigh quality metal housingNumerous software functions, e.g. measurement, focus-stacking (combines several images for more depth of focus), mosaic (for larger image fields)Image and video capture, incl. time-lapse functionGood compatibility with different microscopesHighspeed USB 3.0 connectionModern Sony® IMX226 sensor 7.53 mm x 5.64 mm (1/1.7'')
SYSTEM REQUIREMENTSWindows 10 or 11Intel i3, i5, i7 or i9 CPU from the 6th generation orAMD Ryzen CPUOptical Disk Drive or internet connection for software downloadUSB 3.0, 3.1
SCOPE OF DELIVERYCamera head, USB 3.0 cable 23.2 mm adapter, 30 mm and 30.5 mm adapters capture and image processing software
BRESSER MikroCam PRO HDMI Autofocus
€999.90*
The BRESSER MikroCam Pro HDMI Autofocus delivers excellent image quality with ease of use. The microscopic image is transferred in Full HD resolution and without delay to a HDMI monitor or TV screen. An optional 11" HDMI screen is available under item code 5914110 and can be mounted on the camera directly. It gives brilliant images with high contrast.
Microscope images and video can now be stored on a SD card. The Sony Sensor gives excellent image quality and is very light sensitive. So this camera is recommended for low light contrast methods like darkfield or phase contrast.
The autofocus enables the user to focus without using the mechanical focus wheel. When changing the objective, the focus will automatically be adjusted from the camera. The focus range from the "zero" position (which is the standard backfocus for C-Mount) up to 5,4 mm intrafocal to reach CS-Mount distance, and 10,6 mm extrafocal, to cover a wide range.
In addition to the autofocus functionality, you can set the focus with the mouse or set a user defined focus range.
The camera can be controlled with the included USB mouse directly through the on screen display. The on screen functionality now includes a measuring function as well, so the MikroCam Pro HDMI Autofocus upgrades your existing trinokular microscope to a fully equipped digital autofocus microscope.
With the WiFi module, the camera can also supply the images in a wireless network. Due to this, a permanent connection to a PC via USB is not provided. But the professional software MikroCamLabII is still included with the camera for further image analysis after transferring the data stored on the SD card.
The MikroCam Pro HDMI Autofocus has a wide range of applications:DocumentationAnalysisPresentationQuality ControlAutomationMachine Vision
FEATURESHDMI-connection for a monitor or compatible TVBrilliant images in Full HD using HDMI (60 images / sec) or WiFi (up to 25 images / sec)Direct measuring function using the on screen display (*item no. 5916720 necessary for calibration)Wide dynamic range (WDR)Save images and video on SD card (max. 32 GB, not included)focus range: -5,4 mm bis 0 (C-Mount Abstand) bis 10,6 mm1/4" tripod connectionC-Mount for excellent adaptability, also suitable for C-Mount lenses
SYSTEM REQUIREMENTSWindows 10 or 11Intel i3, i5, i7 or i9 CPU from the 6nd generation orAMD Ryzen CPUOptical Disk Drive or internet connection for software downloadUSB 2.0
SCOPE OF DELIVERYMikroCam Pro HDMI Autofocus with C-MountAC adapterMouseHDMI cableWiFi dongleSoftware (CD-ROM)
MikroCam PRO HDMI camera 5MP
€1,059.90*
The BRESSER MikroCam Pro HDMI delivers excellent image quality with ease of use. The microscopic image is transferred in Full HD resolution and without delay to a HDMI monitor or TV screen. An optional 11'' HDMI screen is available under item code 5914110 and can be mounted on the camera directly. It gives brilliant images with high contrast.
Microscope images and video can now be stored on a SD card with high resolution (up to 5 MP). The Sony Sensor gives excellent image quality.
The camera can be controlled with the included USB mouse directly through the on screen display. The on screen functionality now includes a measuring function as well, so the MikroCam Pro HDMI 5MP upgrades your existing trinokular microscope to a fully equipped digital measuring microscope.
With the WiFi module, the camera can also supply the images in a wireless network. Due to this, a permanent connection to a PC via USB is not provided. But the professional software MikroCamLabII is still included with the camera for further image analysis after transferring the stored on SD card.
Software included in the following languages (for further image analysis - no direct PC connection via USB provided)EnglishGermanFrenchPolishRussianTurkishIndonesianJapaneseChinese (simplified)Chinesisch (traditional)
The software includes numerous functions for image processingmeasurement function* (with live image): distance, angle, surface area (polygon, circle, ellipsoid)Image Stacking from video filesHDR (high dynamic range)fluorescence microscopy: fluorophore data for common dyes can be found in the softwareBatch save
*for the use of the included measurement functionality a object micrometer slide is necessary, e.g. BRESSER item no. 5916720 (1/10mm and 1/100mm) or 5916710 (1/100mm).
FEATURESHDMI-connection on a monitor or TVbrilliant full HD images via HDMI (60 frames / sec) or WiFi (up to 25 frames / sec)measure specimens via on screen display - no PC necessaryWDR (wide dynamic range) function included
SYSTEM REQUIREMENTSWindows 10 or 11Intel i3, i5, i7 or i9 CPU from the 6nd generation orAMD Ryzen CPUOptical Disk Drive or internet connection for software downloadUSB 2.0
SCOPE OF DELIVERYMikroCam Pro HDMI with C-MountAC adapterMouseHDMI cableWiFi dongleSoftware (CD-ROM)
Cover glasses, 200 pcs. 24x50mm Boro 3.3
€17.90*
Extra big cover glasses made from pure white, smooth Borosilicate glass (Boro 3.3). These cover glasses are especially suitable for liquid samples, like plankton or cell culture media. Also useful for skin scrapes from Koi or ornamental fish. You can diagnose a bigger sample volume in one go, making the diagnosis faster and more reliable. The sample will not dry out as fast as usual.Boro 3.3 is a high tech glass material with excellent surface and optical quality. Keep in mind, that the cover glass is not only a cover, but works as an optical element and will influence the resulting image quality. Most microscope objectives are designed for cover glasses of a certain thickness (Usually 0,17mm; marked on the objective).The cover glasses are precleaned and ready to use.Boro 3.3 glass is highly transparent and has excellent surface quality. For UV it is significantly more transparent than soda-lime glass and therefore especially suitable for fluorescence microscopy.SCOPE OF DELIVERY 200 cover glasses 24x50 in plastic box. Incl. drying agent, vacuum sealed.
Cover glasses, 100 pcs. 24x50mm Boro 3.3
€12.90*
Extra big cover glasses made from pure white, smooth Borosilicate glass (Boro 3.3). These cover glasses are especially suitable for liquid samples, like plankton or cell culture media. Also useful for skin scrapes from Koi or ornamental fish. You can diagnose a bigger sample volume in one go, making the diagnosis faster and more reliable. The sample will not dry out as fast as usual.Boro 3.3 is a high tech glass material with excellent surface and optical quality. Keep in mind, that the cover glass is not only a cover, but works as an optical element and will influence the resulting image quality. Most microscope objectives are designed for cover glasses of a certain thickness (Usually 0,17mm; marked on the objective).The cover glasses are precleaned and ready to use.Boro 3.3 glass is highly transparent and has excellent surface quality. For UV it is significantly more transparent than soda-lime glass and therefore especially suitable for fluorescence microscopy.SCOPE OF DELIVERY 100 cover glasses 24x50 in plastic box. Incl. drying agent, vacuum sealed.
BRESSER Immersion oil 5ml nD=1.515
€9.90*
The immersion oil is used as a medium to get focus with a 100x oil objective. Without this oil it's not possible to get a sharp image.Volume: 5 ml
BRESSER Microscope Cutlery Solingen Steel
€67.90*
High-quality dissecting tool set for amateur and professional purposes. Made in Germany!
No matter if amateur microscoper or professional - working with inferior tools is unsatisfying. For that reason, we offer these dissecting tools to you. They are made from Solingen steel and meet the highest quality demands.
Accessories
• 1 Spatula
• 1 Scalpel
• 1 Tweezer
• 1 Scissor
• 1 Slice catcher
• 2 Preparation Needles
• Imitation leather case
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Nexcope NE930 Upright Microscope
€6,850.00*
The NEXCOPE NE930 microscope offers excellent optical performance and ease of use, and is ideal for scientific tasks. Thanks to its cutting-edge optical quality, it is ideal for science and research applications, as well microbiological quality control or diagnostics.
LENSES
The panchromatic lenses ensure a flat field of view.
The backwards-tilted, motorized 6x nosepiece is equipped with high-quality infinity lenses (4x, 10x, 20x, 40x, 100x oil). These feature an M25 thread and a parfocal length of 60 mm. The 40x and 100x lens has a spring-loaded protection mechanism.
EYEPIECES
The ergonomic eyepiece head can be tilted between 0° and 35°. This allows the microscope to be adjusted to the user’s seating position. The eyepieces’ large 25 mm field of view allows you to view a very wide area.
The high eyepiece head is ideal for spectacle wearers. A rubber edge provides protection upon eye contact. A dioptre compensation of -5 to +5 can be set individually for each eyepiece. The interpupillary distance can be adjusted between 47 mm and 78 mm.
ILLUMINATION
The powerful 3 W LED has an adjustable brightness and provides optimal illumination. The brightness can be adjusted individually and saved for each lens. The swing-top condensor is automatically adjusted to the selected lens.
MECHANICS + AUTOMATION
The large XY stage features a robust, chemical-resistant insert made from Gorilla Glass and is moved via a combination of a toothed rack drive and a wire rope hoist. This enables exact positioning for high magnifications. The height of the control knobs can be adjusted to ensure an ergonomic setup. The tension can also be adjusted. Specimens can be conveniently changed with one hand.
In addition to the coarse focus, the fine focus helps to render a very sharp image of the object. A full turn of the fine focus moves the table by 0.2 mm. The fine focus can be adjusted with one finger on the right-hand side. The table height limit helps to protect the specimens. The friction of the coarse focus can also be adjusted.
The fine focus knobs are held in place magnetically and can be replaced as needed.
The lenses are switched via a motor at the touch of a button. The next lens can be moved via a selection switch, or a specific lens can be selected on the front panel. The illumination and the position of the condensor is automatically adjusted to the selected lens. These features ensure ergonomic, fatigue-free and efficient performance. The exact reproducibility of the settings also offers advantages for photomicrography, as the images do not need to be recalibrated / flats do not need to be reproduced each time the lens is changed.
CAMERA CONNECTION
The NEXCOPE NE930 is specially designed for use with C-mount cameras. You can choose from two different adapters, depending on the sensor size and desired field of view:
0.5x c-mount adapter for sensor sizes up to 2/3’’
1x c-mount adapter for sensor sizes of 1’’
As the adapter can be adjusted, the camera image can always be exactly matched parfocally to the eyepieces.
ACCESSORIES (optional)
Dark field condenser (dry or oil)
Phase contrast
Differential interference contrast (DIC)Fluorescence
Polarisation
FEATURES
Professional, motorised transmitted light microscope
Lens type: Planachromatic (60 mm parfocal length)
Precise mechanics
Outstanding expandability
Bright LED illumination
WHAT’S INCLUDED
Microscope stand with five planachromatic lenses (4x, 10x, 20x, 40x, 100x oil)
One pair of 10x eyepieces (field of view: 25 mm, Plug-in diameter: 30 mm), diopter adjustmentDust protection cover
Mains cable